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Ion exchange chromatography can be used either for a separate purification step or for a second polishing purification step after affinity chromatography purification as an orthogonal chemistry to obtain a highly pure final product. The result is complete protein capture and high final protein concentrations. Due to the physiological buffers used during the protocol, ion exchange chromatography is a generally gentle purification method.
Ion Exchange PhyTip® columns is used for efficient parallel purification of small protein samples.
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PT PACIFIC SCIENTIFIC INDONESIA
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