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Proteinase K

IDR0
-+
Code: Biochemicals
Brand: Vivantis
Category: > Molecular and Microbiology Reagent
Tags: Proteinase K
Description:

Grade
Molecular Biology Grade

Description
Proteinase K is a recombinant enzyme that expressed in Pichia pastoris which is originally isolated from the mold Tritirachium album. It is highly active, subtilisin-related serine endopeptidases that does not exhibit any pronounced cleavage specificity. Thus, Proteinase K is treated as a universal tool for nucleic acid template preparation.

The recombinant form and native protease are having identical amino acid sequence and molecular structure. Compared to native protease, the recombinant enzyme guarantees an enzyme of outstanding reliability and purity meeting all requirements of molecular and diagnostic tests. The recombinant form of Proteinase K enzyme maximizes the yield of target nucleic acids. The lyophilized form as well as the solution form experience stability at room temperature and flexibility during shipment at room temperature.

Applications

- Digest native proteins very efficiently.

- Inactivate endogenous RNases and DNases rapidly during nucleic acid isolation.

- Isolation of native RNA and DNA from tissues and cell lines.

- Promotes cell lysis by activating a bacterial autolytic factor.

- Analysis of membrane structures by modifying proteins and glycoproteins on cell surfaces.

- Removal of cellular debris during the preparation of colony lifts.

- Treatment of tissue sections to ensure efficient probe infiltration during in situ hydridization.

Unit Definition of Protenaise K (lyophilized form)
Approximately 50U/ml solution (chromozyme assay); approximately 600U/ml solution (hemoglobin assay). One unit is the enzyme activity which cleaves at 25°C in 1 min 18mmol Chromozym TRY (equivalent to 600U/ml with the hemoglobin assay).

Specification of Proteinase K (lyophilized form)

Specification of Proteinase K (solution form)

Storage
Store at -20°C for long shelf life
Store at 4°C and ambient temperature for short shelf life up to 1 year

Ordering Information




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