×
Products Brands
0
0 IDR0
Your shopping cart is empty!

Ribonuclease A (RNase A)

IDR0
-+
Code: Biochemicals
Brand: Vivantis
Category: > Molecular and Microbiology Reagent
Tags: Ribonuclease A (RNase A)
Description:

Grade
Molecular Biology Grade

Description
RNase A is an endoribonuclease that is from bovine pancreas for molecular biology applications. The major application for RNase A is the removal of RNA from preparation of plasmid DNA as well as extraction of plasmid DNA. It is also used in removal of unspecifically bound RNA; RNase protection assays; analysis of RNA sequences as well as hydrolysis of RNA contained in protein samples.

RNase A attacks at the 3¢ phosphate of a pyrimidine nucleotide. The sequence of pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG. The highest activity is exhibited with single stranded RNA. RNase A is a single chain polypeptide containing 4 disulfide bridges. RNase A can be inhibited by alkylation of His12 or His119, which are present in the active site of the enzyme. Activators of RNase A include potassium and sodium salts.

Source
Bovine pancreas
All products from animal sources are produced from starting material of USDA-approved origin, collected in United States Department of Agriculture (USDA) or equivalent approved facilities, inspected to be free of disease and suitable for exportation. Certificates of Origin are available upon request.

Unit Definition
One unit according to Kunitz produces an increase in absorbance of 0.001/minute under assay conditions in 1ml at 260nm.

Specification

Notes
For the application of removal of RNA from preparations of plasmid DNA, the suggested final concentration used is 0.2 mg/mL. Boiling stock solutions of the RNase A to inactivate residual DNase I is not necessary and may cause precipitation of RNase A and possible loss of enzymatic activity. If the RNase A solution is heated at a neutral pH, precipitation will occur. If the RNase A solution heated at a lower pH, some precipitation may occur due to the protein impurities that are present.

Storage
Store at -20°C

Ordering Information


© 2015-2025 PACIFIC SCIENTIFIC | Laboratorium Equipment Distributor. All Rights Reserved.